{"id":1004,"date":"2013-03-21T11:49:20","date_gmt":"2013-03-21T15:49:20","guid":{"rendered":"http:\/\/sites.biochem.umass.edu\/vierlinglab\/?page_id=1004"},"modified":"2017-10-31T10:28:07","modified_gmt":"2017-10-31T14:28:07","slug":"eman-basha-visiting-scientist-june-13-august-13","status":"publish","type":"page","link":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/eman-basha-visiting-scientist-june-13-august-13\/","title":{"rendered":"Dr. Eman Basha,  Senior Research Scientist, University of Arizona Tucson"},"content":{"rendered":"<p><a href=\"http:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2013\/03\/eman.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-medium wp-image-1005\" src=\"https:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2013\/03\/eman-270x300.jpg\" alt=\"\" width=\"270\" height=\"300\" srcset=\"https:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2013\/03\/eman-270x300.jpg 270w, https:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2013\/03\/eman-922x1024.jpg 922w, https:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2013\/03\/eman-600x665.jpg 600w\" sizes=\"auto, (max-width: 270px) 100vw, 270px\" \/><\/a><\/p>\n<p>My research is focused on investigating the function of the two major classes of cytosolic sHSPs, class I and II, in\u00a0<em>Arabidopsis thaliana<\/em>. I created transgenic plants with increased (constitutive) or decreased (by RNAi) expression levels of either Class I or Class II cytosolic sHSPs. I also generated plants expressing affinity-tagged versions of these two sHSPs, regulated either by the CaMV 35S promoter or the sHSP\u2019s own, heat-inducible promoter. I isolated and identified proteins that showed heat-specific association with either the Class I or Class II sHSPs. I am also studying how sHSPs recognize substrate in the model genetic organism\u00a0<em>Synechocystis sp. PCC 6803<\/em>. To achieve that I use wild type or mutant sHSPs to determine how N-terminal arm mutations impact native substrate binding and processing in vivo.<\/p>\n<p>I am also interested in studying the role of sHSP dynamics in substrate binding using NMR. To achieve that I am producing uniformly labeled\u00a0<sup>2<\/sup>H sample of TaHsp16.9 and PsHsp18.1 prepared with NMR active\u00a0<sup>13<\/sup>CH<sub>3<\/sub>\u00a0labels at Ile-delta1, Val gamma1\/gamma2 and Leu delta1\/delta2.\u00a0 I am hoping also to study the dynamic information on the substrate by using labeled substrate in the presence of unlabeled sHSP.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>My research is focused on investigating the function of the two major classes of cytosolic sHSPs, class I and II, in\u00a0Arabidopsis thaliana. I created transgenic plants with increased (constitutive) or decreased (by RNAi) expression&#46;&#46;&#46;<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-1004","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages\/1004","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/comments?post=1004"}],"version-history":[{"count":7,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages\/1004\/revisions"}],"predecessor-version":[{"id":2187,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages\/1004\/revisions\/2187"}],"wp:attachment":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/media?parent=1004"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}