{"id":199,"date":"2011-12-07T11:18:57","date_gmt":"2011-12-07T16:18:57","guid":{"rendered":"http:\/\/sites.biochem.umass.edu\/vierlinglab\/?page_id=199"},"modified":"2012-01-19T14:00:17","modified_gmt":"2012-01-19T19:00:17","slug":"dr-eman-basha","status":"publish","type":"page","link":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/vierling-lab-personnel\/dr-eman-basha\/","title":{"rendered":"Dr. Eman Basha"},"content":{"rendered":"<p><a href=\"http:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2011\/12\/eman.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright  wp-image-654\" src=\"https:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2011\/12\/eman.jpg\" alt=\"\" width=\"430\" height=\"478\" \/><\/a><\/p>\n<p>telephone: 520-780-7608<\/p>\n<p>Email: basha&#8217;a&#8217;email.arizona.edu<\/p>\n<p>My research is focused on investigating the function of the two major classes of cytosolic sHSPs, class I and II, in <em>Arabidopsis thaliana<\/em>. I created transgenic plants with increased (constitutive) or decreased (by RNAi) expression levels of either Class I or Class II cytosolic sHSPs. I also generated plants expressing affinity-tagged versions of these two sHSPs, regulated either by the CaMV 35S promoter or the sHSP\u2019s own, heat-inducible promoter. I isolated and identified proteins that showed heat-specific association with either the Class I or Class II sHSPs. I am also studying how sHSPs recognize substrate in the model genetic organism <em>Synechocystis sp. PCC 6803<\/em>. To achieve that I use wild type or mutant sHSPs to determine how N-terminal arm mutations impact native substrate binding and processing in vivo.<\/p>\n<p>I am also interested in studying the role of sHSP dynamics in substrate binding using NMR. To achieve that I am producing uniformly labeled <sup>2<\/sup>H sample of TaHsp16.9 and PsHsp18.1 prepared with NMR active <sup>13<\/sup>CH<sub>3<\/sub> labels at Ile-delta1, Val gamma1\/gamma2 and Leu delta1\/delta2.\u00a0 I am hoping also to study the dynamic information on the substrate by using labeled substrate in the presence of unlabeled sHSP.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>telephone: 520-780-7608 Email: basha&#8217;a&#8217;email.arizona.edu My research is focused on investigating the function of the two major classes of cytosolic sHSPs, class I and II, in Arabidopsis thaliana. I created transgenic plants with increased (constitutive)&#46;&#46;&#46;<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":7,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-199","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages\/199","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/comments?post=199"}],"version-history":[{"count":10,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages\/199\/revisions"}],"predecessor-version":[{"id":521,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages\/199\/revisions\/521"}],"up":[{"embeddable":true,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages\/7"}],"wp:attachment":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/media?parent=199"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}