{"id":3268,"date":"2024-10-02T20:54:36","date_gmt":"2024-10-03T00:54:36","guid":{"rendered":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/?page_id=3268"},"modified":"2024-10-09T13:37:55","modified_gmt":"2024-10-09T17:37:55","slug":"nicholas-cassidy","status":"publish","type":"page","link":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/nicholas-cassidy\/","title":{"rendered":"Nicholas Cassidy"},"content":{"rendered":"\n<p>My project aims to understand the function of ATAD3 genes in&nbsp;<em>Arabidopsis thaliana<\/em>. I am working on identifying homozygous single-knockout mutants of the ATAD3 genes, which were constructed using CRISPR-Cas9.&nbsp;In another project, I am generating transgenic plants with amiRNA-mediated reduction of one ATAD3 gene in the background of a single knockout mutant of a different ATAD3 gene. This approach creates a partial loss-of-function mutant, addressing the challenge posed by the lack of phenotype in single and double-knockout ATAD3 mutants, which limits genetic analysis.<\/p>\n\n\n\n<p>ncassidy@umass.edu<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2024\/10\/Nick.jpg\"><img loading=\"lazy\" decoding=\"async\" width=\"576\" height=\"1024\" src=\"https:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2024\/10\/Nick-576x1024.jpg\" alt=\"\" class=\"wp-image-3272\" srcset=\"https:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2024\/10\/Nick-576x1024.jpg 576w, https:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2024\/10\/Nick-169x300.jpg 169w, https:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2024\/10\/Nick-768x1365.jpg 768w, https:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2024\/10\/Nick-864x1536.jpg 864w, https:\/\/sites.biochem.umass.edu\/vierlinglab\/files\/2024\/10\/Nick.jpg 1080w\" sizes=\"auto, (max-width: 576px) 100vw, 576px\" \/><\/a><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>My project aims to understand the function of ATAD3 genes in&nbsp;Arabidopsis thaliana. I am working on identifying homozygous single-knockout mutants of the ATAD3 genes, which were constructed using CRISPR-Cas9.&nbsp;In another project, I am generating&#46;&#46;&#46;<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-3268","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages\/3268","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/comments?post=3268"}],"version-history":[{"count":2,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages\/3268\/revisions"}],"predecessor-version":[{"id":3273,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/pages\/3268\/revisions\/3273"}],"wp:attachment":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/media?parent=3268"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}