{"id":764,"date":"2012-05-30T10:52:23","date_gmt":"2012-05-30T14:52:23","guid":{"rendered":"http:\/\/sites.biochem.umass.edu\/vierlinglab\/?p=764"},"modified":"2012-05-30T10:58:49","modified_gmt":"2012-05-30T14:58:49","slug":"new-article-in-chemistry-and-biology","status":"publish","type":"post","link":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/2012\/05\/30\/new-article-in-chemistry-and-biology\/","title":{"rendered":"New Article in Chemistry and Biology"},"content":{"rendered":"

Chemistry and Biology, <\/strong>25 May, 2012 Volume 19, Issue 5<\/p>\n

Dissecting Heterogeneous Molecular Chaperone<\/strong>
\nComplexes Using a Mass Spectrum<\/strong>
\nDeconvolution Approach<\/strong>
\nFlorian Stengel,1,6,8 Andrew J. Baldwin,2,8 Matthew F. Bush,1,7 Gillian R. Hilton,1 Hadi Lioe,1 Eman Basha,3,4 Nomalie Jaya,4 Elizabeth Vierling,5 and Justin L.P. Benesch1,*
\n1\u00a0 Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, Oxford OX1 3QZ, UK
\n2\u00a0 Departments of Molecular Genetics, Biochemistry, and Chemistry, University of Toronto, Toronto, Ontario M5S 1A1, Canada
\n3\u00a0 Botany Department, Faculty of Science, Tanta University, Tanta, Gharbia 31111, Egypt
\n4\u00a0 Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721, USA
\n5\u00a0 Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA
\n6\u00a0 Present address: Institute of Molecular Systems Biology, ETH Zu\u00a8 rich, CH-8093 Zurich, Switzerland
\n7\u00a0 Present address: Department of Chemistry, University of Washington, Seattle, WA 91895, USA
\n8\u00a0 These authors contributed equally to this work
\n*Correspondence: justin.benesch@chem.ox.ac.uk
\nDOI 10.1016\/j.chembiol.2012.04.007<\/p>\n

SUMMARY<\/strong>
\nSmall heat-shock proteins (sHSPs) are molecular chaperones that prevent irreversible aggregation through binding nonnative target proteins. Due to
\ntheir heterogeneity, these sHSP:target complexes remain poorly understood. We present a nanoelectrospray mass spectrometry analysis algorithm
\nfor estimating the distribution of stoichiometries comprising a polydisperse ensemble of oligomers.\u00a0 We thus elucidate the organization of complexes
\nformed between sHSPs and different target proteins. We find that binding is mass dependent, with the resultant complexes reflecting the native quaternary
\narchitecture of the target, indicating that protection happens early in the denaturation. Our data therefore explain the apparent paradox of how variable
\ncomplex morphologies result from the generic mechanism of protection afforded by sHSPs. Our approach is applicable to a range of polydisperse proteins and provides a means for the automated and accurate interpretation of mass spectra derived
\nfrom heterogeneous protein assemblies.<\/p>\n

For Complete Article and Supporting Material:<\/p>\n

StengelChemBiol2012<\/a>StengelSupportinginfoChemBiol2012<\/a><\/p>\n

2012 Dissecting Heterogeneous Molecular Chaperone Complexes using a Mass <\/a><\/p>\n

Spectrum Deconvolution Approach – Feature2012<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Chemistry and Biology, 25 May, 2012 Volume 19, Issue 5 Dissecting Heterogeneous Molecular Chaperone Complexes Using a Mass Spectrum Deconvolution Approach Florian Stengel,1,6,8 Andrew J. Baldwin,2,8 Matthew F. Bush,1,7 Gillian R. Hilton,1 Hadi Lioe,1...<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-764","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/posts\/764","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/comments?post=764"}],"version-history":[{"count":4,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/posts\/764\/revisions"}],"predecessor-version":[{"id":770,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/posts\/764\/revisions\/770"}],"wp:attachment":[{"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/media?parent=764"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/categories?post=764"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sites.biochem.umass.edu\/vierlinglab\/wp-json\/wp\/v2\/tags?post=764"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}