Personnel – Amherst
Dr. Elizabeth Vierling – Principal Investigator – Rm 329N LSL
ORCID number: https://orcid.org/0000-0002-0066-4881
https://scholar.google.com/citations?hl=en&authuser=1&user=1dMWBMsAAAAJ
https://www.researchgate.net/profile/Elizabeth_Vierling
Administrative Assistant
Rebecca Fricke – Rm 335N LSL
Postdoctoral Researchers – 340N LSL
Patrick Treffon – I am analyzing the biochemical properties of the GSNOR protein, including protein-protein interactions and their impact on NO-dependent signaling. The functional characterization of gsnor– knock out plants is also one focus of my research.
Undergraduates
Katelyn Belmore – Working on creating TRAP and WT mutants for the ATAD3 homologue A2 to trap interacting substrates. I am also screening CRISPR-Cas and quick change mutagenesis mutant plants for the transgene. My goal is to foster healthy and happy plants that yield transgenic seeds! (Began Spring 2023)
Nicholas Cassidy – Working to understand the function of ATAD3 genes in Arabidopsis thaliana and working to identify homozygous single-knockout mutants of the ATAD3 genes, which were constructed using CRISPR-Cas9. In another project, Nick is generating transgenic plants with amiRNA-mediated reduction of one ATAD3 gene in the background of a single knockout mutant of a different ATAD3 gene. This approach creates a partial loss-of-function mutant, addressing the challenge posed by the lack of phenotype in single and double-knockout ATAD3 mutants, which limits genetic analysis. (Began Summer of 2024)
Haofeng Lin – Working on the two classes of cytosolic small heat shock proteins (sHSPs) (Class I and II). His goals for both are to perform phenotypic analysis in order to know the impact of heat shock protein on plants. For CI he hopes to accomplish 6 mutant lines and for CII I he hopes to perform at one set of phenotypic analysis. (Began Spring 2023)
Alina Shkurikhina – Alina Shkurikhina – Working on characterizing null mutant and complemented lines of the chloroplast small heat shock protein (sHSP). This project aims to shed light on the function of this conserved protein through phenotypic analysis. I am also working on studying the potential of GFP fusion constructs for rescuing normally lethal double mutants of ATAD3. Additionally, I am applying my skills as a mechanical engineering double major at the lab by designing an imaging chamber that will be used to quantify plant phenotypes. (Began Fall 2023).
Nicole Steinmeyer – Working to understand nitric oxide (NO) signaling in plants by studying the regulation of GSNOR, S-nitrosoglutathione reductase, which regulates NO levels. My work aims to validate protein-protein interactions with GSNOR using bimolecular fluorescence complementation (BiFC) methodology. I have focused on thioredoxins (TRX), a class of redox proteins, as potential interactors with GSNOR. I am also working to purify GSNOR protein to perform further tests to validate GSNOR-protein interactions. (Began Spring 2023)
High School
Conall Gahan – Working to identify both mutant and complemented lines of CI and CII small heat shock proteins (sHSPs) in Arabidopsis thaliana. Hoping to further understand the function of sHSPs in plants. (Began Summer 2024)
Former Lab Members
Visiting Research Scientists:
Dr. Alejandra Covarrubias – Biotechnology Institute of the National University of Mexico (Universidad Nacional Autonoma de Mexico – UNAM
Dr. Eman Basha– Associate Professor at the Biology Department, Taif University, Ta’if Saudi Arabia (June ’13 – August ’13)Kishor
Dr. Gaikwad, Research Scientist, Indian Agricultural Research Institute, Pusa, New Delhi, India
Dr. Huiru Peng, Professor, China Agricultural University, Beijing, China.
Dr. Joel Stafstrom, Associate Professor of Plant Sciences, NIU, IL
Dr. Shengbao Xu, Associate professor in Northwest A&F University of China
Research Fellow –
Dr. Minsoo Kim
Post Docs:
Dr. Ricardo Azpiroz, Instructor, Richland College, Richland, TX
Dr. Kim Giese, RN Princeton, NJ
Dr. Damian Guerra, Post Doc, University of Colorado Denver
Dr. Andy Hausrath, Assistant Professor, UA
Dr. Ken Helm, Professor, Siena College, NY.
Dr. Indu Santhanagoplan – PhD Indian Institute of Science, India.
Dr. Suk-Whan Hong, Associate Professor, Chonnam National Univ., Gwngju, South Korea
Dr. Jane Larkindale, Research Program Coordinator, Muscular Dystrophy Association, Tucson, AZ
Dr. Gary Lee former Monsanto Scientist,currently fine guitar builder, http://www.leeguitarworks.com/
Dr. Ung Lee, True Light Institute of Agricultural Development in Northeast Asia, Yan Ji, China.
Dr. Fionn McLoughlin, Postdoctoral research fellow in Richard Vierstra’s lab at Washington University in St. Louis
Dr. Heather O’Neil, Caris Life Sciences
Dr. Kathy Osteryoung, Professor Michigan State University, MI
Dr. Kerstin Petersen
Dr. Elizabeth Waters, Associate Professor, San Diego State University, CA
Dr. Colin Watson, Medical Director at GE Health Care in Tucson, AZ
PhD Students:
Dr. Keith Ballard – Small heat shock proteins (sHSPs) and related vertebrate α-crystallins are a ubiquitous class of ATP-independent molecular chaperones with a monomeric molecular mass of 12-42 kDa.
Dr.Qiang Chen, Associate Professor, Arizona State University, Tempe, AZ
Dr. Amy DeRocher, Staff Scientist, Seattle Biomedical Research Institute, Seattle, WA
Dr. Kenneth Friedrich, Associate Professor, Portland Community College, Portland, OR
Tienxiang Liu – Northwest Agriculture and Forestry University, Yangling, China
Dr. Nomalie Jaya, Senior Scientist, Seattle Genetics, Bothell, WA
Dr. Teri Suzuki, Research Scientist-Sanofi-Aventis, Tucson, AZ
Dr. Nadja Wehmeyer (Anderson), Biotech Project Coordinator, Tucson, AZ
MS Students:
Eli Gordon – Graduated Fall 2021
Sam Zelman – Scientist III at Merck Research Labs Boston in the Immuno-oncology Department
Parth Patel – University of Central Florida Medical School
Nathen Bopp – University of TX, PhD program
Mary Fowler – New England Biolabs
David Kim High School Science Teacher, Albuquerque, NM
Deanna Willett, MIddle School Science Teacher and Teacher Training, Tucson, AZ
Ian Truebridge – How nitrosation and pH affects the activity of S-(hydroxymethyl) glutathione reductase (S-HMGSH)
Yichen Zhang, Rutgers University
Liyuan Zhang – China Agricultural University, Beijing, China.
Research Assistant
Salma Akther
Research Technicians:
Stephanie Craig
Angela Blais
Lisa Lauzon
Adam Geach, DMD, California
Sharon Pennington
Julie Bordowitz
Susanna Brettschneider Student Umea, Sweden,
Debbie Raynes – Senior research Technician, Tucson, AZ
Former Undergraduates:
Aurora Ahmed, Jesse Arsenault, Umaru Barrie, Johnathan Barricelli, Julie Bordowitz, Thi Bui,Elana Carleton,J. T. Caroll, MD, Belmund Catague (MD), Jennifer Corrigan (PharmD), Samuel Del’Olio, Mindy Escobar (MS), Olena Gross, Philipp Guettler, Nora Haggerty, Lorraine Hernandez, Senior Research Scientist, Erica Hummell (Pharmacist), Corey Isgur, Yadwinder Kuar, Samhitha Satish Kumar, Lisa Miller, Dylan McLaughlin, Alyssa McQuillan, Chirag Mehta, Jillian Moore, Esther Oh, Shannon Parrington, Parth Patel, Prem Patel, Vijay Patel (Dentist), Megan Paul (MD), Kostantinos Papathanasiou, Emma Peterson, Benjamin Rizkin, Astha Parmar, Fabian Suri-Payer, Puneet Singh, Ariel Toledo (Pharmacist), Elli Walker (Dialysis Technician), Chris Wie (MD), Ben Williams (MD), Justin Wong (MD), Kelsey Avery, Logan Robinson, Kara Samsel (MD), Lauren Velie
Former High School Students:
Elizabeth Ortiz, Amherst High School
Zoe Langsdale, Amherst High School
CG Elliott, Amherst High School